DU4475 (ATCC® HTB-123)

Organism: Homo sapiens, human  /  Tissue: mammary gland/breast  / 

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue mammary gland/breast
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Age 70 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 93; range = 85 to 95.
The cell line is aneuploid human female, with chromosome counts in the tetraploid range. Normal chromosomes N16 and N21 are least well represented, with from none to two copies per karyotype, while chromosome N7 tends to be over-represented, with four or five copies per karyotype, whereas two to four copies are found of most of the remaining normal chromosomes. Twenty-two marker chromosomes are found including: iso(1q), del(1)(q32), der(1)t(1;?)(p36;?), del(3)(q24), der(3)t(3;?)(q24;?), del(3)(p14p25), t(6q;21?) and others. Eight are identical to those reported or portrayed in the report by A.J. Langlois, et al.
Clinical Data
70 years
Caucasian
female
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
Numerous desmosomes and interdigitating microvilli were observed by transmission electron microscopy.
This line grows in floating clusters and viability cannot be determined accurately with dye exclusion tests.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%


Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (RefBerthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc. Natl. Acad. Sci. USA 83: 2496–2500, 1986. PubMed: 3458212). The Phenol Red-free version of RPMI 1640, is ATCC catalog No. 30-2602.

Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 2 X 105 cells/mL and maintain at between 105 and 106 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,12
D13S317: 11,14
D16S539: 11,12
D5S818: 11
D7S820: 9,10
THO1: 6,8
TPOX: 8
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1-2
PGM3, 1
Name of Depositor AJ Langlois
References

Langlois AJ, et al. Morphological and biochemical properties of a new human breast cancer cell line. Cancer Res. 39: 2604-2613, 1979. PubMed: 221108

Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

The cells form transplantable tumors similar in histology to that of the original carcinoma.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Langlois AJ, et al. Morphological and biochemical properties of a new human breast cancer cell line. Cancer Res. 39: 2604-2613, 1979. PubMed: 221108

Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

The cells form transplantable tumors similar in histology to that of the original carcinoma.