WBC264-9C (ATCC® HB-8902)

Organism: human (leukocyte); mouse (macrophage)  /  Cell Type: Macrophage,Macrophage-like  /  Tissue: peripheral blood  / 

Organism human (leukocyte); mouse (macrophage)
Tissue peripheral blood
Cell Type Macrophage,Macrophage-like
Product Format frozen
Morphology macrophage
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:  BALB/c (macrophage)

Applications
This is a macrophage-like cell line derived by fusion of normal human peripheral blood leukocytes with the mouse macrophage cell line, RAW 264.
The cells exhibit chemotaxis to N-formyl-methionine-leucine-phenylalanine (fMet-Leu-Phe, FMLP).
Storage Conditions liquid nitrogen vapor temperature
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype at passage 6, the mean number of chromosome was 64 with a range of 36 to 81
Derivation
This is a macrophage-like cell line derived by fusion of normal human peripheral blood leukocytes with the mouse macrophage cell line, RAW 264.
Comments
This is a macrophage-like cell line derived by fusion of normal human peripheral blood leukocytes with the mouse macrophage cell line, RAW 264.
The cells exhibit chemotaxis to N-formyl-methionine-leucine-phenylalanine (fMet-Leu-Phe, FMLP).
WBC264-9 cells express human HPRT.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Subcultures are prepared by scraping. DO NOT USE TRYPSIN! Remove old medium, add fresh, dislodge cells and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor The United States of America
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Aksamit RR. Human-mouse hybrid cell line expressing monocyte-macrophage properties. US Patent 4,970,162 dated Nov 13 1990

Aksamit RR. A human-mouse hybrid cell line that stably expresses chemotaxis to N- formylmethionyl-leucyl-phenylalanine. Biochem. Biophys. Res. Commun. 138: 1001-1008, 1986. PubMed: 3741419

Basic Documentation
References

Aksamit RR. Human-mouse hybrid cell line expressing monocyte-macrophage properties. US Patent 4,970,162 dated Nov 13 1990

Aksamit RR. A human-mouse hybrid cell line that stably expresses chemotaxis to N- formylmethionyl-leucyl-phenylalanine. Biochem. Biophys. Res. Commun. 138: 1001-1008, 1986. PubMed: 3741419