51.1 (ATCC® HB-230)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an antigen associated with human cytotoxic/suppressor T cells (CD8).
The antibody strongly inhibits cytotoxic T lymphocyte function and cross-reacts with peripheral blood T cells from chimpanzee, orangutan, gibbon, baboon, macaque, capuchin monkey, squirrel monkey and spider monkey.
Derivation
Spleen cells were fused with NSI/1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD8
Cellular Products
immunoglobulin; monoclonal antibody; against human CD8
Comments
Animals were immunized with human peripheral blood T lymphocytes.
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with an antigen associated with human cytotoxic/suppressor T cells (CD8).
The antibody strongly inhibits cytotoxic T lymphocyte function and cross-reacts with peripheral blood T cells from chimpanzee, orangutan, gibbon, baboon, macaque, capuchin monkey, squirrel monkey and spider monkey.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Complete Growth Medium
Culture Medium: RPMI medium 1640 with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate suppelmented with 50 ?M 2-mercaptoethanol, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Isotype IgG2a
Name of Depositor PJ Martin
Deposited As mouse (B cell); mouse (myeloma)
References

Martin PJ, et al. Epitope mapping of the human surface suppressor/cytotoxic T cell molecule Tp32. J. Immunol. 132: 759-765, 1984. PubMed: 6197474

Basic Documentation
References

Martin PJ, et al. Epitope mapping of the human surface suppressor/cytotoxic T cell molecule Tp32. J. Immunol. 132: 759-765, 1984. PubMed: 6197474