Attention:

We will be conducting system maintenance starting on Friday, December 13th, at 6:00 p.m. (ET) through Sunday, December 15th. During this time, many normal functions normally performed on our website, such as placing an order or paying an invoice, will not be available. We apologize for the inconvenience but will have complete return of services on Monday, December 16th.
X

MD2 (ATCC® HB-229)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
In the mouse thymus, MD2 is specific for dendritic cells in the medulla.
The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.
It also reacts with epithelial elements in a variety of mouse tissues.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium
Cellular Products
immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium
Comments
Animals were immunized with mouse thymic stromal tissue.
Spleen cells were fused with Sp2/0 myeloma cells.
In the mouse thymus, MD2 is specific for dendritic cells in the medulla.
The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.
It also reacts with epithelial elements in a variety of mouse tissues.
Complete Growth Medium RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: RPMI medium 1640 with non-essential amino acids, 1 mM sodium
pyruvate and 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor RV Rouse
Deposited As rat (B cell); mouse (myeloma)
References

Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413

Basic Documentation
References

Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413