Mouse hybridoma MAb 951-5-1 (P450 1A2) (HB-12684)

Designation / Description Mouse hybridoma MAb 951-5-1 (P450 1A2)
U.S. Patent Number
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Animals were immunized with baculovirus-expressed human cytochrome P450 1A2 [U.S. Pat. 6,335,428]. Spleen cells were fused ? MAb 26-7-5 (ATCC HB-12681), MAb 951-5-1 (ATCC HB-12684) and MAb 1812-4-8 (ATCC HB-12683) specifically bind to human cytochrome P450 1A2. They inhibit 1A2 enzyme activity [U.S. Pat. 6,335,428]. None of the three MAbs cross-react with human cytochromes 1A1, 2A6, 2B6, 2C8, 2C9, 2C18, 2C19, 2D6, 2E1, 3A4, and 3A5 as measured by ELISA and immunoblotting procedures. The antibodies exhibit strong immunoblotting to 1A2 [U.S. Pat. 6,335,428]. The antibodies can be used for screening drugs for metabolism by cytochrome P450 1A6, and in methods of measuring p450 1A2 levels in individuals relative to p450 1A2 levels in a control population [U.S. Pat. 6,335,428].
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Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Basic Documentation
References

Gelboin HV, et al. Agents that bind to and inhibit human cytochrome P450 1A2. US Patent 6,335,428 dated Jan 1 2002

Yang TJ, et al. Inhibitory monoclonal antibodies to human cytochrome P450 1A2: analysis of phenacetin O-deethylation in human liver. Pharmacogenetics 8: 375-382, 1998. PubMed: 9825829