NCI-BL2195 [BL2195] (ATCC® CRL-5956™) Organism: Homo sapiens, human / Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed / Tissue: peripheral blood / Disease: normal General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits View Restrictions Organism Homo sapiens, human Tissue peripheral blood Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 2 [Cells contain Herpesvirus] Disease normal Age 67 years Gender male Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Clinical Data 67 years Caucasian male Comments A small cell lung cancer cell line from this patient is available as ATCC CRL-5931. Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 106 viable cells/mL. Maintain cell density between 1 x 106 and 3 x 106 cells/mL. Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density). Cryopreservation Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. Culture Conditions Temperature: 37°C STR Profile Amelogenin: X,YCSF1PO: 11D13S317: 11D16S539: 10,14D5S818: 11,12D7S820: 10,11THO1: 9.3TPOX: 8,9vWA: 17,18 Name of Depositor AF Gazdar, JD Minna References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS Restrictions The line is available with the following restrictions: This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.