NCI-H774 [H774] (ATCC® CRL-5842)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: soft tissue  /  Disease: stage E, carcinoma; classic small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: soft tissue
Product Format frozen
Morphology floating aggregates of round cell clusters
Culture Properties suspension
Biosafety Level 1
Disease stage E, carcinoma; classic small cell lung cancer
Age 43 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype del(p13-p21), del(p11-pter), del(p13-pter)
Derivation
The line was established in July 1984.
Clinical Data The patient was a smoker
male
Caucasian
43 years old
30 pack years
Comments
Fast growing.

Complete Growth Medium HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters. Single cells are dead and clusters are viable.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).
Cryopreservation
Culture medium, 90%; DMSO, 5%; extra FBS, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 8
D16S539: 12
D5S818: 11
D7S820: 9,11
THO1: 6,9.3
TPOX: 8
vWA: 15,17
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1984
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The line is available with the following restrictions: 

  1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 
  2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.