NCI-H727 [H727] (ATCC® CRL-5815)

Organism: Homo sapiens, human  /  Tissue: lung, bronchus  /  Disease: carcinoid

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Organism Homo sapiens, human
Tissue
lung, bronchus
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease carcinoid
Age 65 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from tissue taken prior to therapy.
Clinical Data
65 years
Caucasian
female
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from tissue taken prior to therapy.
Receptor Expression
epidermal growth factor (EGF)
Genes Expressed
neuromedin B (NMB)
Comments
This is the best differentiated of the available bronchial carcinoid lines.
The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung.
The cells are able to synthesize the peptide NMB (at 0.1 pmol/mg protein), but not the gastrin releasing peptide (GRP).
The cell line secretes a parathyroid hormone-like protein which is calcium stimulated through a protein kinase C pathway.
Growth of NCI-H727 cells is inhibited by epidermal growth factor (EGF) receptor monoclonal antibodies.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing div>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 14,15
Name of Depositor AF Gazdar, JD Minna
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Brandt DW, et al. Calcium-stimulated parathyroid hormone-like protein secretion: potentiation through a protein kinase-C pathway. Endocrinology 128: 2999-3004, 1991. PubMed: 2036974

Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Natl. Cancer Inst. Monogr. 13: 117-123, 1992.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Brandt DW, et al. Calcium-stimulated parathyroid hormone-like protein secretion: potentiation through a protein kinase-C pathway. Endocrinology 128: 2999-3004, 1991. PubMed: 2036974

Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Natl. Cancer Inst. Monogr. 13: 117-123, 1992.