23 ScCr [10ScNCr/23] (ATCC® CRL-2751)

Organism: Mus musculus, mouse  /  Cell Type: macrophage  /  Tissue: bone marrow  /  Disease: FIV

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Organism Mus musculus, mouse
Tissue bone marrow
Cell Type macrophage
Product Format frozen
Morphology macrophage
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease FIV
Age 6 months adult
Strain C57BL/10ScN (formerly C57BL/10ScNCr, C57BL/10ScCr)
Storage Conditions liquid nitrogen vapor phase
Derivation
23 ScCr is a bone marrow derived macrophage cell line developed from a mouse strain with a deletion of the TLR4 locus.
Antigen Expression
CD11a, mouse; Mus musculus
CD11b, mouse; Mus musculus
F4/80, mouse
Genes Expressed

Macrophage inflammatory protein-2

tumor necrosis factor alpha (TNF alpha)

Comments

The cell line is deficient in toll-like receptor 4 (TLR4). Stimulation with lipoteichoic acid (LTA) induces a five to six fold increase in tumor necrosis factor alpha (TNF alpha) and macrophage inflammatory protein-2 (MIP-2) secretion. No increases in either cytokine were found when cells were stimulated with lipopolysaccharides (LPS).

Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Subculturing
Remove 75% of the media. Scrape off the attached cells. Resuspend cells at 2 x 105 viable cells/mL. Transfer into new flasks.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor E Lorenz
References

Lorenz E, et al. Toll-like receptor 4 (TLR4)-deficient murine macrophage cell line as an in vitro assay system to show TLR4-independent signaling of Bacteroides fragilis lipopolysaccharide. Infect. Immun. 70: 4892-4896, 2002. PubMed: 12183533

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lorenz E, et al. Toll-like receptor 4 (TLR4)-deficient murine macrophage cell line as an in vitro assay system to show TLR4-independent signaling of Bacteroides fragilis lipopolysaccharide. Infect. Immun. 70: 4892-4896, 2002. PubMed: 12183533

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm