202-11A8 (ATCC® CRL-2669)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type:: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic polypeptide with the sequence LGQGCFGEVWMG corresponding to peptides 276 to 287 of the SRC/YES oncogene (formerly 273 to 284).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic SRC/YES oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic SRC/YES oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence LGQGCFGEVWMG corresponding to peptides 276 to 287 of the SRC/YES oncogene (formerly 273 to 284).
Spleen cells were fused with Sp2/0 myeloma cells.
Using immunoblot techniques, the antibody recognizes proteins of 140,000, 70,000, 60,000 and 55,000 daltons.
The IgG1 reactivity of the antibody with the src peptide (276-287) was confirmed by ELISA.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1 and IgG2b
Name of Depositor National Cancer Institute