GDM-1 (ATCC® CRL-2627)

Organism: Homo sapiens, human  /  Cell Type:: monoblast  /  Tissue: peripheral blood  /  Disease: myelomonoblastic leukemia

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type monoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease myelomonoblastic leukemia
Age 66 - 67 years
Gender female
Ethnicity White
Applications
This cell line is a model system for studying myelomonocytic disorders and leukemias.
Storage Conditions liquid nitrogen vapor temperature
Derivation
The GDM-1 cell line was established in 1980 from the peripheral blood of a patient with a Philadelphia chromosome negative myeloproliferative disorder, after transformation to acute myelomonoblastic leukemia.
Clinical Data
66 - 67 years
female
White
Antigen Expression
Ia +; myeloid leukemia antigen (M-1)
Receptor Expression
complement (C3)
Fc
Genes Expressed
lysozyme
myeloperoxidase
Cellular Products
lysozyme
myeloperoxidase
Tumorigenic No
Effects
No, did not form colonies in soft agar
Comments
The cells lack B- and T-cell surface markers including T-associated antigens, E-rosetting capacity, surface and intracytoplasmic immunoglobulins. They are non-specific esterase positive.
The cells are phagocytic for latex beads and iron particles. Exposure to phorbol 12-myristate 13-acetate (TPA) increases the phagocytic activity. TPA also induces macrophage-like differentiation.
The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 x 10 5 viable cells/mL. Maintain cultures at cell concentrations between 3 x 10 5 and 3 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).

Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C

Population Doubling Time 24 to 36 hrs
Name of Depositor H Ben-Bassat
Year of Origin 1980
References

Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552

Basic Documentation
References

Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552