H36.12j [Pixie 12j] (ATCC® CRL-2449)

Organism: Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage)  /  Cell Type: Macrophage  / 

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Organism Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage)
Cell Type Macrophage
Product Format frozen
Morphology macrophage
Culture Properties suspension (some adherent cells)
Biosafety Level 1
Applications
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
The cells produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation and serve as an in vitro model for chronic beryllium disease in humans.
Since intracellular Listeria escape into the cytoplasm where they replicate, the cell line is a useful tool in the study of the permissive intracellular growth cycle of Listeria.
These cells may be used in assays for the phagocytosis and killing of microorganisms, in assays for the production of cytokines by mouse macrophages, and for assays for the effect of environmental toxins on mouse macrophages.
Derivation
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
Genes Expressed
surface Interleukin-10 (Interleukin 10; IL-10),tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation
Comments
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
H36.12j is a model for the permissive intracellular growth of Listeria monocytogenes within mouse macrophages. The cells phagocytose Listeria via receptors for internalin A (InlA), a Listeria surface polypeptide.
The cells produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation and serve as an in vitro model for chronic beryllium disease in humans.
Since intracellular Listeria escape into the cytoplasm where they replicate, the cell line is a useful tool in the study of the permissive intracellular growth cycle of Listeria.
The H36.12j cells have been found to express biologically active surface IL-10 that regulates macrophage bacterial activity.
These cells may be used in assays for the phagocytosis and killing of microorganisms, in assays for the production of cytokines by mouse macrophages, and for assays for the effect of environmental toxins on mouse macrophages.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated iron supplemented bovine calf serum to a final concentration of 10%
  • Subculturing
    Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
    Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
    Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
    Adherent cells may be harvested by scraping.
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37.0°C
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Name of Depositor RT Sawyer
    Year of Origin 1991
    References

    Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.

    Fleming SD, Campbell PA. Macrophages have cell surface IL-10 that regulates macrophage bactericidal activity. J. Immunol. 56: 1143-1150, 1996. PubMed: 8557991

    Sawyer RT, et al. Internalin A can mediate phagocytosis of Listeria monocytogenes by mouse macrophage cell lines. J. Leukocyte Biol. 60: 603-610, 1996. PubMed: 8929551

    Fleming SD, Campbell PA. Some macrophages kill Listeria monocytogenes while others do not. Immunol. Rev. 158: 69-77, 1997. PubMed: 9314075

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.

    Fleming SD, Campbell PA. Macrophages have cell surface IL-10 that regulates macrophage bactericidal activity. J. Immunol. 56: 1143-1150, 1996. PubMed: 8557991

    Sawyer RT, et al. Internalin A can mediate phagocytosis of Listeria monocytogenes by mouse macrophage cell lines. J. Leukocyte Biol. 60: 603-610, 1996. PubMed: 8929551

    Fleming SD, Campbell PA. Some macrophages kill Listeria monocytogenes while others do not. Immunol. Rev. 158: 69-77, 1997. PubMed: 9314075