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H36.12d [Pixie 12d] (ATCC^® CRL-2447^™)

Organism: Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage) / Cell Type: Macrophage /

H36.12d [Pixie 12d] ATCC^® CRL-2447^™

frozen

For-Profit: $551.00 Non-Profit: $459.17

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Permits and Restrictions	View Permits
Organism	Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage)
Cell Type	Macrophage
Product Format	frozen
Morphology	macrophage
Culture Properties	mixed: adherent and suspension
Biosafety Level	1
Applications	This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells. Unlike H36.12j (ATCC CRL-2449), the cells do not produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation.

Derivation	This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
Comments	This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells. Unlike H36.12j (ATCC CRL-2449), the cells do not produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated iron supplemented bovine calf serum to a final concentration of 10%
Subculturing	Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 - 4 day is recommended Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 X 10exp5 viable cells/ml.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C

Name of Depositor	RT Sawyer
Year of Origin	1991
References	Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
References	Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.