Complete Growth Medium
|
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
heat-inactivated iron supplemented bovine calf serum to a final concentration of 10%
|
Subculturing
|
Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 - 4 day is recommended Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 X 10exp5 viable cells/ml.
|
Cryopreservation
|
culture medium 95%; DMSO, 5%
|