BL3.1 (ATCC® CRL-2306)

Organism: Bos taurus, cow  /  Cell Type: B lymphocyte  /  Disease: lymphosarcoma

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Organism Bos taurus, cow
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphosarcoma
Age 3 month old
Gender male
Strain Hereford
Applications
BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3. The BL-3 cell line was isolated from a 3-month-old male Hereford calf.
BL3.1 cells are a major histocompatibility complex (MHC) transcriptional loss variant; the cells exhibit no expression of MHC class I and high expression of MHC class II. Few cell lines express only MHC class II.
The cell line is positive for bovine leukemia virus (BLV) and actively produces BLV.
CRL-2306 can be used in BLV and retroviral studies and as a target for NK assays.
Derivation
BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3. The BL-3 cell line was isolated from a 3-month-old male Hereford calf.
Clinical Data
BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3. The BL-3 cell line was isolated from a 3-month-old male Hereford calf.
male
Genes Expressed
CCD 1105 KIDTr is sensitive to Coxsackieviruses A9 and B5, measles virus, poliovirus 1 and rhinovirus.
bovine leukemia virus (BLV),BL3.1 cells are a major histocompatibility complex (MHC) transcriptional loss variant; the cells exhibit no expression of MHC class I and high expression of MHC class II. Few cell lines express only MHC class II.
Cellular Products
bovine leukemia virus (BLV)
Comments
BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3. The BL-3 cell line was isolated from a 3-month-old male Hereford calf.
BL-3 cells were exposed to gamma-irradiation and surviving cells were immunoselected for MHC class I antigen loss.
BL3.1 cells are a major histocompatibility complex (MHC) transcriptional loss variant; the cells exhibit no expression of MHC class I and high expression of MHC class II. Few cell lines express only MHC class II.
The cell line is positive for bovine leukemia virus (BLV) and actively produces BLV.
CRL-2306 can be used in BLV and retroviral studies and as a target for NK assays.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JS Harms
Year of Origin 1990
References

Harms JS, Splitter GA. Impairment of MHC class I transcription in a mutant bovine B cell line. Immunogenetics 35: 1-8, 1992. PubMed: 1345904

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Harms JS, Splitter GA. Impairment of MHC class I transcription in a mutant bovine B cell line. Immunogenetics 35: 1-8, 1992. PubMed: 1345904