NR8383 [AgC11x3A, NR8383.1] (ATCC® CRL-2192)

Organism: Rattus norvegicus, rat  /  Cell Type: macrophage (alveolar)  /  Tissue: lung  /  Disease: normal

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Organism Rattus norvegicus, rat
Tissue lung
Cell Type macrophage (alveolar)
Product Format frozen
Morphology macrophage
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease normal
Strain Sprague-Dawley
Applications
The NR8383 cell line provides a homogenous source of highly responsive alveolar macarophages which can be used in vitro to study macrophage related activities.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
NR8383 (normal rat, August 3, 1983) was established from normal rat alveolar macrophage cells obtained by lung lavage.
NR8383 cells were cloned and subcloned from single cells by limiting dilution, and then subcloned from soft agar three times.
Receptor Expression
Fc
Genes Expressed
transforming growth factor beta (TGF beta); interleukin 1 (IL-1); interleukin 6 (IL-6)
Comments
The cells were cultured in the presence of gerbil lung cell conditioned medium for approximately 8 to 9 months.
Subsequently the requirement for exogenous growth factors was lost.
The cells exhibit characteristics of macrophage cells.
Phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst,IL-1, TNF beta and IL-6 secretion, and replicative response to exogenous growth factors.
The cells respond to appropriate microbial, particulate or soluble stimuli with phagocytosis and killing.
NR8383 cells respond to bleomycin by secreting latent transforming growth factor (TGF beta).
Stimulation with bleomycin also increases TGF beta mRNA expression.
These cells are sensitive to endotoxin.
LPS levels of 1 to 10 ng/mL inhibit replication by 50%.
LPS inhibition is nontoxic and reversible even after levels up to 0.001 mg/mL for extended periods.
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; heat inactivated fetal bovine serum, 15%
Subculturing Cultures can be maintained by transferring floating cells to additional flasks. Adherent cells may be harvested by scraping. Upon reseeding, about one half of the cells will re-attach. Cultures are most successful when set up at a floating cell concentration of 1- 4 x 105 viable cells/mL.

Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor RJ Helmke
Year of Origin August 3, 1983
References

Hidalgo HA, et al. Pneumocystis carinii induces an oxidative burst in alveolar macrophages. Infect. Immun. 60: 1-7, 1992. PubMed: 1729174

Helmke RJ, et al. A continuous alveolar macrophage cell line: comparisons with freshly derived alveolar macrophages. In Vitro Cell. Dev. Biol. 25: 44-48, 1989. PubMed: 2914814

Helmke RJ, et al. From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell. Dev. Biol. 23: 567-574, 1987. PubMed: 3497918

Limper AH, Standing JE. Vitronectin interacts with Candida albicans and augments organism attachment to the NR8383 macrophage cell line. Immunol. Lett. 42: 139-144, 1994. PubMed: 7534269

Hidalgo HA, et al. The effects of cyclosporine and dexamethasone on an alveolar macrophage cell line (NR8383). Transplantation 53: 620-623, 1992. PubMed: 1549855

Denholm EM, Rollins SM. Expression and secretion of transforming growth factor-beta by bleomycin-stimulated rat alveolar macrophages. Am. J. Physiol. 264: L36-L42, 1993. PubMed: 7679254

Krieg DP, et al. Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect. Immun. 56: 3173-3169, 1988. PubMed: 3141284

Sherman MP, et al. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem. Biophys. Res. Commun. 191: 1301-1308, 1993. PubMed: 7682068

Griscavage JM, et al. Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. J. Immunol. 151: 6329-6337, 1993. PubMed: 7504017

Henderson SA, et al. Nitric oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-gamma and lipopolysaccharide. J. Biol. Chem. 269: 25239-25242, 1994. PubMed: 7523382

Huang S, et al. Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts. Biochem. Biophys. Res. Commun. 184: 922-929, 1992. PubMed: 1374243

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Hidalgo HA, et al. Pneumocystis carinii induces an oxidative burst in alveolar macrophages. Infect. Immun. 60: 1-7, 1992. PubMed: 1729174

Helmke RJ, et al. A continuous alveolar macrophage cell line: comparisons with freshly derived alveolar macrophages. In Vitro Cell. Dev. Biol. 25: 44-48, 1989. PubMed: 2914814

Helmke RJ, et al. From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell. Dev. Biol. 23: 567-574, 1987. PubMed: 3497918

Limper AH, Standing JE. Vitronectin interacts with Candida albicans and augments organism attachment to the NR8383 macrophage cell line. Immunol. Lett. 42: 139-144, 1994. PubMed: 7534269

Hidalgo HA, et al. The effects of cyclosporine and dexamethasone on an alveolar macrophage cell line (NR8383). Transplantation 53: 620-623, 1992. PubMed: 1549855

Denholm EM, Rollins SM. Expression and secretion of transforming growth factor-beta by bleomycin-stimulated rat alveolar macrophages. Am. J. Physiol. 264: L36-L42, 1993. PubMed: 7679254

Krieg DP, et al. Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect. Immun. 56: 3173-3169, 1988. PubMed: 3141284

Sherman MP, et al. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem. Biophys. Res. Commun. 191: 1301-1308, 1993. PubMed: 7682068

Griscavage JM, et al. Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. J. Immunol. 151: 6329-6337, 1993. PubMed: 7504017

Henderson SA, et al. Nitric oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-gamma and lipopolysaccharide. J. Biol. Chem. 269: 25239-25242, 1994. PubMed: 7523382

Huang S, et al. Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts. Biochem. Biophys. Res. Commun. 184: 922-929, 1992. PubMed: 1374243