LCL 8664 (ATCC® CRL-1805)

Organism: Macaca mulatta, monkey, rhesus  /  Cell Type: B lymphocyte  /  Disease: lymphoma

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Organism Macaca mulatta, monkey, rhesus
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Disease lymphoma
Age 5 years
Gender male
Applications
CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard.
Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.
Clinical Data
male
Genes Expressed
B lymphotropic herpesvirus
Comments
CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard.
Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.
About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp5 cells/ml and maintain between 4 X 10 exp5 and 2 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor SR Rangan
References

Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986. PubMed: 3017870

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986. PubMed: 3017870