LCL 8664 (ATCC® CRL-1805)

Organism: Macaca mulatta, monkey, rhesus  /  Cell Type: B lymphocyte  /  Disease: lymphoma

Permits and Restrictions

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Organism Macaca mulatta, monkey, rhesus
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Age 5 years
Gender male
Applications
CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard.
Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.
Clinical Data
male
Genes Expressed
B lymphotropic herpesvirus
Cellular Products
B lymphotropic herpesvirus
Comments
CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard.
Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.
About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp5 cells/ml and maintain between 4 X 10 exp5 and 2 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor SR Rangan
References

Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986. PubMed: 3017870

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986. PubMed: 3017870