45.6.TG1.7 (ATCC® CRL-1608)

Organism: Mus musculus, mouse  /  Cell Type: B lymphoblast  /  Disease: plasmacytoma; myeloma

Organism Mus musculus, mouse
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Gender female
Strain BALB/c
Applications
The cells are resistant to 6-thioguanine (HPRT -).
Tested and found negative for ectromelia virus (mousepox).
Clinical Data
female
Antigen Expression
H-2d
Genes Expressed
immunoglobulin,H-2d
Comments
The cells are resistant to 6-thioguanine (HPRT -).
The line was cured at the ATCC of a mycoplasma infection.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml.
Isotype IgG2b; kappa light chain
Name of Depositor MD Scharff
References

Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836

Margulies DH, et al. Somatic cell hybridization of mouse myeloma cells. Cell 8: 405-415, 1976. PubMed: 821618

Basic Documentation
References

Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836

Margulies DH, et al. Somatic cell hybridization of mouse myeloma cells. Cell 8: 405-415, 1976. PubMed: 821618