HS-Sultan (ATCC® CRL-1484)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: Burkitt's lymphoma

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, multicell aggregates
Biosafety Level 2 [Cells contain EBV DNA viral sequences]

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Burkitt's lymphoma
Age 7
Gender male
Ethnicity Black, African
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hyperdiploid human cell line. The modal chromosome number was 48, occurring in 80% of cells, and the rate of polyploid cells was 3.4%. Three marker chromosomes are common to all cells. M1 and M2 [der(14)t(8;14) (q24;q32)] were probably derived from the balanced translocation between del(8) (p21.1;p21.3) and N14. The third marker, M3, is ?der(8)t(8;?) (p21.1;?). Most cells had three copies of N7. Both normal X and Y were clearly identifiable.
Derivation
Although deposited with the ATCC as a plasmacytoma cell line, DNA fingerprinting has shown this line to be a derivative of Jiyoye (ATCC CCL-87), a Burkitt's lymphoma cell line.
Clinical Data
Black, African
male
7
Antigen Expression
CD11a +; CD19 +; CD20 +; CD28 -; CD38 +; CD49e +
Genes Expressed
immunoglobulin
Cellular Products
immunoglobulin
Comments
The cells are EBNA positive (EBNA +).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 105 viable cells/mL. Maintain cell density between 3 X 105 and 3 X 106 viable cells/mL. Do not allow the cell density to exceed 4 X 106 cells/mL.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
Isotype kappa light chain
Name of Depositor NS Harris
References

Harris NS. Plasma cell surface antigen on human blood lymphocytes. Nature 250: 507-509, 1974. PubMed: 4549735

. . J. Immunol. 118: 1480-1482, 1976.

. . Proceedings of the Seventh Leukocyte Culture Conference : 15-28, 1973.

Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Harris NS. Plasma cell surface antigen on human blood lymphocytes. Nature 250: 507-509, 1974. PubMed: 4549735

. . J. Immunol. 118: 1480-1482, 1976.

. . Proceedings of the Seventh Leukocyte Culture Conference : 15-28, 1973.

Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375