RBL-1 (ATCC® CRL-1378)

Organism: Rattus norvegicus, rat  /  Cell Type: chemically-induced basophil  /  Tissue: peripheral blood  /  Disease: leukemia

Organism Rattus norvegicus, rat
Tissue peripheral blood
Cell Type chemically-induced basophil
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease leukemia
Strain Wistar
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Receptor Expression
FcERI (Fc of IgE)
Comments
This cell line exhibits various characteristics of basophil differentiation including surface receptors for IgE. It was shown to not release histamine by an IgE mediated system. RefBarsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
 Cultures can be maintained by the addition or replacement of fresh medium. Start cultures at 2 x 105 viable cells/mL and maintain between 1 x 105 cells/mL and 1 x 106 /mL.
Medium Renewal: Add medium as cell density increases
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor H Metzger, C Isersky
References

Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481

Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973.

Cross References

Nucleotide (GenBank) : NM_031580 Rattus norvegicus glucose regulated protein, 58 kDa (Grp58), mRNA.

Nucleotide (GenBank) : X12355 Rat mRNA for phosphoinositide-specific phospholipase C form-I (PI-PLC I).

Basic Documentation
References

Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481

Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973.