FeT-1C (ATCC® CRL-11968)

Organism: Felis catus, cat  /  Cell Type: T lymphocyte  /  Tissue: peripheral blood mononuclear cells (PBMC); T lymphocyte  / 

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Organism Felis catus, cat
Tissue peripheral blood mononuclear cells (PBMC); T lymphocyte
Cell Type T lymphocyte
Product Format frozen
Biosafety Level 1
Derivation
The cell line was derived from a specific pathogen free (SPF) cat [U.S. Pat. 5,846,825].
Virus Susceptibility Feline immunodeficiency virus
Comments

The FeT-1C cell line (ATCC CRL-11968) is IL-2 dependent, whereas the FeT-J cell line (ATCC CRL-11967) is IL-2 independent [U.S. Pat. 5,846,825] This feline T-cell line is susceptible to infection by feline immunodeficiency virus (FIV) [U.S. Pat. 5,846,825].


Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100 Units/ml human recombinant IL-2 and 10% heat-inactivated fetal bovine serum
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor University of Florida, Gainesville
References

Yamamoto JK. Feline derived T-cell lines for producing FIV. US Patent 5,846,825 dated Dec 8 1998

Hohdatsu T, et al. Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence. J. Gen. Virol. 77: 93-100, 1996. PubMed: 8558132

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Yamamoto JK. Feline derived T-cell lines for producing FIV. US Patent 5,846,825 dated Dec 8 1998

Hohdatsu T, et al. Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence. J. Gen. Virol. 77: 93-100, 1996. PubMed: 8558132