Amdur II (ATCC® CCL-124)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Permits and Restrictions

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 1 year
Gender male
Ethnicity Caucasian
Karyotype normal human male; stable
Clinical Data
male
Caucasian
1 year
Comments
The cells have defect in propionic acid metabolism and accumulate methylmalonic acid.
The cells have a life expectancy of at least 40 serial passages.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 24 hours after thawing; from then on at time of subculture
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and allow the culture to incubate at 37C for about 10 minutes or until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 7 days.
Isoenzymes
G6PD, B
Name of Depositor WJ Mellman
Passage History
The cells have a life expectancy of at least 40 serial passages.
References

Morrow G, et al. Propionate metabolism in cells cultured from a patient with methylmalonic acidemia. Pediatr. Res. 3: 217-219, 1969. PubMed: 5788784

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Morrow G, et al. Propionate metabolism in cells cultured from a patient with methylmalonic acidemia. Pediatr. Res. 3: 217-219, 1969. PubMed: 5788784