Detroit 573 (ATCC® CCL-117)

Organism: Homo sapiens, human  /  Cell Type:: fibroblast, cytogenetic abnormality  / 

Organism Homo sapiens, human
Cell Type fibroblast, cytogenetic abnormality
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age newborn
Gender female
Ethnicity Caucasian
Karyotype B/D translocation; 45,XX,t(Bp-;Dq+); translocation involves chromosomes 5 and 14
Clinical Data
female
Caucasian
newborn
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Isoenzymes
G6PD, B
Name of Depositor CS Stulberg