Detroit 548 (ATCC® CCL-116)

Organism: Homo sapiens, human  /  Cell Type: fibroblast, cytogenetic abnormality  / 

Organism Homo sapiens, human
Cell Type fibroblast, cytogenetic abnormality
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age infant
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype partial D group trisomy; 46,XX,C+,D-; C+ results from D - D translocation
Clinical Data
female
Caucasian
infant
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, sodium pyruvate (1 mM) and lactalbumin hydrolysate (0.1%), 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:11 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isoenzymes
G6PD, B
Name of Depositor CS Stulberg
References

Zuelzer WW, et al. Evidence for a genetic factor related to leukemogenesis and congenital anomalies: chromosomal aberrations in pedigree of an infant with partial D trisomy and leukemia. J. Pediatr. 72: 367-376, 1968. PubMed: 5237794

Basic Documentation
References

Zuelzer WW, et al. Evidence for a genetic factor related to leukemogenesis and congenital anomalies: chromosomal aberrations in pedigree of an infant with partial D trisomy and leukemia. J. Pediatr. 72: 367-376, 1968. PubMed: 5237794