K32 [pUC-Tag1436] (ATCC® 87351)

Organism: Triticum aestivum Linnaeus, wheat  /  Clone Type: Clone  /  Depositors: RD Thompson

Permits and Restrictions

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Designations K32 [pUC-Tag1436]
Species Triticum aestivum Linnaeus, wheat
Depositors RD Thompson
contains sequence gliadin, gamma
Construct size (kb): 4.0
Insert lengths(kb): 1.299999952316284
Gene product: gliadin, gamma [Gli-1]
Target Gene: gliadin, gamma
Insert Size (kb) 1.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): PstI--2.7, 1.3; HindIII--2.8, 1.2; SalI--2.9, 1.1.

Bartels D, et al. Molecular analysis of c-gliadin gene families at the complex Gli-1 locus of bread wheat (T. aestivum L.). Theor. Appl. Genet. 72: 845-853, 1986.