p70 [W3110/p70] (ATCC® 69334)

Organism: Clostridium histolyticum (Weinberg and Seguin) Bergey et al.  /  Clone Type: Clone  /  Depositors: H Lin, MS Nasoff, H Lin

Permits and Restrictions

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Designations p70 [W3110/p70]
Species Clostridium histolyticum (Weinberg and Seguin) Bergey et al.
Depositors H Lin, MS Nasoff, H Lin
Applications
Encodes the 68kD form of C. histolyticum collagenase, which may be useful in isolating islets from the human pancreas for clinical islet transplantation.
Insert
DNA: genomic
Insert lengths(kb): 1.700000047683716
Gene product: collagenase (68kD form)
Insert Size (kb) 1.700
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--23.0, 4.4.
Encodes the 68kD form of C. histolyticum collagenase, which may be useful in isolating islets from the human pancreas for clinical islet transplantation.
References

Lin HC, Lei SP. Recombinant 68,000 dalton collagenase of Clostridium histolyticum. US Patent 5,418,157 dated May 23 1995

Bascic Documentation
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Product Sheet
Product Sheet
Product Sheet