pCF1EF+C33c [D1210 (pCF1EF+C33c)] (ATCC® 67953)

Organism: Hepatitis C virus  /  Clone Type: Clone  /  Depositors: Chiron Corp., L Chung, Chiron Corp.

Permits and Restrictions

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Designations pCF1EF+C33c [D1210 (pCF1EF+C33c)]
Species Hepatitis C virus
Depositors Chiron Corp., L Chung, Chiron Corp.
Applications
expresses antigenic determinant protease nonstructural protein 3
Vector
Construct size (kb): 4.0
Insert
DNA: cDNA
Insert lengths(kb): 0.8199999928474426
Gene product: protease(nonstructural protein 3) [NS3]
Target Gene: protease
Insert Size (kb) 0.820
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped:  Freeze dried  E. coli containing the plasmid.

Comments
Expression of the SOD/HCV fusion protein is inducible by IPTG.
The hepatitis C virus (HCV) insert encodes amino acids 1192 - 1457 of the HCV polyprotein.
The pSODCF1 vector was modified by removing the small BamHI/EcoRI fragment (thus losing the internal SmaI site), and then inserting a linker that restored the BamHI and EcoRI sites in the appropriate reading frame, and introduced a downstream AflII site.
References

Houghton M, et al. HCV immunoassays employing C domain antigens. US Patent 5,350,671 dated Sep 27 1994

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Bascic Documentation
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.