pRLalpha1M/101 (ATCC® 63108)

Organism: Rattus norvegicus, rat  /  Clone Type: Clone  /  Depositors: GH Fey

Permits and Restrictions

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Designations pRLalpha1M/101
GenBank Number

M77183

Species Rattus norvegicus, rat
Depositors GH Fey
Vector
Construct size (kb): 7.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 4.900000095367432
Tissue: liver, acute phase
Gene product: alpha-1-macroglobulin [A1M]
Insert Size (kb) 4.900
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): SstI--5.6, 2.35; HindIII--4.9, 3.0; EcoRI--4.4, 3.45, 0.5; BamHI--3.0, 2.6, 1.5, 0.8.
Digestion of the plasmid with BamHI yields a 3.0 kb fragment from the vector and fragments of 2.6, 0.8, and 1.5 kb from the insert. The 2.6 and 1.5 kb fragments contain fewer than 100 bp from the vector.
The insert contains the following restriction sites (nt position according to the published sequence): BamHI--2541, 3346; HindIII--2720.
Contains the complete coding sequence and 3' untranslated region and part of the 5' untranslated region.
References

Sottrup-Jensen L, et al. The alpha-macroglobulin bait region. Sequence diversity and localization of cleavage sites for proteinases in five mammalian alpha-macroglobulins. J. Biol. Chem. 264: 15781-15789, 1989. PubMed: 2476433

Enghild JJ, et al. alpha-Macroglobulin from Limulus polyphemus exhibits proteinase inhibitory activity and participates in a hemolytic system. Biochemistry 29: 10070-10080, 1990. PubMed: 1703001

Eggertsen G, et al. Sequence of rat alpha1-macroglobulin, a broad-range proteinase inhibitor from the alpha-macroglobuin-complement family. Mol. Biol. Med. 8: 287-302, 1991. PubMed: 1725450

Georg H Fey, personal communication

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