CRI-R191 (ATCC® 61736)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: Collaborative Research, Inc.

Permits and Restrictions

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Designations CRI-R191
Species Homo sapiens, human
Depositors Collaborative Research, Inc.
Construct size (kb): 44.5
DNA: genomic
Insert lengths(kb): 14.0
Tissue: liver
Gene product: DNA Segment, single copy [D8S26]
Alleles: A2, B1, B2, B3, B4, A1, A3
Insert Size (kb) 14.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Restriction digests of the clone give the following sizes (kb): BamHI--26.0, 7.4, 5.8, 3.8; EcoRI--20.0, 11.0, 8.6, 4.2, 1.3; XbaI--20.0, 15.0, 3.8, 3.1; KpnI--20.0, 17.0, 3.0, 1.4; HindIII--22.0, 7.8, 6.6, 5.0, 1.6.
The MspI and TaqI polymorphisms have a combined PIC value of 0.67 and a combined heterozygosity of 76%.
Enzyme(s) not detecting polymorphism: BglII, PstI, HindIII, EcoRI, RsaI, HincII (panel of 5 individuals).

Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396

Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638