cTBIRBP-9 (ATCC® 59986)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White, Y Nakamura

Permits and Restrictions

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Designations cTBIRBP-9
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Construct size (kb): 48.0
DNA: genomic
Insert lengths(kb): 40.0
Gene product: retinol-binding protein 3, interstitial(retinol-binding protein 3, interstitial (includes former D10S64, D10S65, D10S66)) [RBP3]
Alleles: A2, B2, A1, A2, A2, A2, B2, B2, B2, A1, A1, A1, A1, A1, A1, A2, A2, A2, B1, B1, B2, C1, D1, D2, A1, A2, A3, C2, A1, A1, A1, A1, A1, A1, A1, A1, A1, A2, A2, A2, A2, A2, A2, A2, A2, A2, B1, B1, B1, B1, B1, B1, B2, B2, B2, C1, C1, C1, D1, D1, D1, D2, D2, D2, A1, A1, A1, A2, A2, A2, A3, A3, A3, C2, C2, C2
Insert Size (kb) 40.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): EcoRI--12.0, 8.4, 6.0, 4.4, 2.15, 1.65, 1.25, 1.2, 0.87, 0.8; BamHI--11.0, 8.8, 7.8, 5.5, 2.6, 2.15, 1.9, 1.7; HindIII--9.4, 8.4, 6.6, 4.4, 3.7, 3.1, 2.2, 1.9, 1.6;
PstI--3.5, 3.0, 2.5, 2.3, 2.2, 2.1 + smaller; XhoI--31.0, 7.8, 0.78.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Enzyme(s) not detecting polymorphism: PstI.

Nakamura Y, et al. Linked markers flanking the gene for multiple endocrine neoplasia type 2A. Genomics 5: 199-203, 1989. PubMed: 2571570

White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325

Yusuke Nakamura, personal communication