pJW101 (ATCC® 57601)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pJW101
GenBank Number


Species Homo sapiens, human
Construct size (kb): 6.349999904632568
Insert lengths(kb): 0.6000000238418579
Gene product: hemoglobin, alpha 1( alpha 1 globin, globin, alpha 1) [HBA1]
Insert Size (kb) 0.600
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Restriction digests of the clone give the following sizes (kb): EcoRI--similar to uncut; HindIII--5.8, 0.65; BamHI--6.4; AvaI--4.7, 1.65; SmaI--uncut.
The actual cDNA insert is about 600 bp. It cannot be excised as such because of AT tailing, but can be excised as a 1.6 kb MboII fragment containing 845 bp of pMB9.
HindIII and MboII patterns of ATCC preparation match that of Forget's isolate.
HindIII site 275 bp 3' to AT/exon 1 joint (in exon 2); 0.7 kb HindIII fragment contains 3' end of gene; 5.2 kb fragment contains 5' end; TaqI site 130 bp 5' to HindIII site.
The 5' end at the insert coincides exactly with the ATG initiation site, 275 bp from the internal HindIII site. It lacks all the 5' untranslated sequences and the NcoI site bridging the initiation site.

Wainscoat JS, et al. Association of two DNA polymorphisms in the alpha globin gene cluster. Am. J. Hum. Genet. 35: 1086-1089, 1983. PubMed: 6316779

Wilson JT, et al. Insertion of synthetic copies of human globin genes into bacterial plasmids. Nucleic Acids Res. 5: 563-581, 1978. PubMed: 345245

Wilson JT, et al. Nucleotide sequence of the coding portion of human alpha globin messenger RNA. J. Biol. Chem. 255: 2807-2815, 1980. PubMed: 6244294

Bernard G Forget, personal communication