Permits and Restrictions

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Designation / Description PGL2C PLASMID IN ESCHERICHIA COLI JM83
U.S. Patent Number
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): BamHI--7.0; BglII--7.0, EcoRI--4.6, 1.8, 0.33.
One of 3 vectors (pGL2A ATCC 53365, pGL2B 53366, pGL2C ATCC 53367) differing in the length of a synthetic linker and thus providing alternate reading frames.
Constructed by inserting a HindIII/BglII fragment containing the glucoamylase gene into pUC12. A BssHII/SstI fragment (starting just 3' to the region coding the signal peptide) was then replaced with a synthetic linker having EcoRV and BglII sites.
Shipped freeze-dried
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation

Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993