Monosiga gracilis Kent (ATCC® 50964)

Strain Designations: BSA-02190019  /  Depositor: TA Nerad  /  Biosafety Level: 1

Strain Designations BSA-02190019
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
salt marsh sediment, Cattleshed Marsh, eastern shore of Virginia, 2000
Product Format frozen
Type Strain no
Medium ATCC® Medium 1525: Seawater 802 medium
Growth Conditions
Temperature: 25.0°C
Duration: grown with Klebsiella pneumoniae subsp. pneumoniae ATCC 700831 and mixed bacteria
Protocol: To subcultivate, virgorously agitate a T-25 flask at or near peak density and aseptically transfer a 0.5-ml aliquot to a fresh T-25 flask containing 10 ml of bacterized ATCC medium 1525.
Subcultivation
Protocol: To subcultivate, virgorously agitate a T-25 flask at or near peak density and aseptically transfer a 0.5-ml aliquot to a fresh T-25 flask containing 10 ml of bacterized ATCC medium 1525.
Name of Depositor TA Nerad
Year of Origin 2000
References

Thomas A Nerad, personal communication