Reclinomonas sp. (ATCC® 50633)

Strain Designations: H-2  /  Depositor: AP Mylnikov, T Cavalier-Smith  /  Biosafety Level: 1

Permits and Restrictions

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Strain Designations H-2
Biosafety Level 1
Isolation
freshwater pond, Yaroslavl, Russia, 1981
Product Format frozen
Type Strain no
Medium ATCC® Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25.0°C
Protocol: ATCCNO: 50633 SPEC: The strain is routinely distributed as a frozen stabilate. Thaw the ampule and aseptically transfer the material to a T-25 tissue culture flask containing 10 ml of ATCC medium 802. The baterial flora that is present in the shipped culture will support growth. Growth may be improved by using bacterized ATCC medium 802 with a single species of bacteria. Bacterization is accomplished by inoculating the medium with Enterobacter aerogenes ATCC 13048 approximately 24 hours before use. Other species of bacteria may work equally well but this must be empirically assessed. The culture is routinely passaged every 14 days. Although this strain does form cysts they are not stable for long periods. To subculture, agitate and aseptically transfer 0.25 ml to 10 ml of freshly bacterized medium in a T-25 flask. Screw the cap on tightly and incubate at 25C.
Subcultivation
Protocol: ATCCNO: 50633 SPEC: The strain is routinely distributed as a frozen stabilate. Thaw the ampule and aseptically transfer the material to a T-25 tissue culture flask containing 10 ml of ATCC medium 802. The baterial flora that is present in the shipped culture will support growth. Growth may be improved by using bacterized ATCC medium 802 with a single species of bacteria. Bacterization is accomplished by inoculating the medium with Enterobacter aerogenes ATCC 13048 approximately 24 hours before use. Other species of bacteria may work equally well but this must be empirically assessed. The culture is routinely passaged every 14 days. Although this strain does form cysts they are not stable for long periods. To subculture, agitate and aseptically transfer 0.25 ml to 10 ml of freshly bacterized medium in a T-25 flask. Screw the cap on tightly and incubate at 25C.
Name of Depositor AP Mylnikov, T Cavalier-Smith
Year of Origin 1981
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation