Miamiensis avidus Thompson and Moewus (ATCC® 50180)

Organism: Miamiensis avidus Thompson and Moewus  /  Depositor: AT Soldo, EB Small

Strain Designations Ma/2
Biosafety Level 1
Product Format test tube
Type Strain no
Comments
Oral morphogenesis
Prey-induced transformation
Medium ATCC® Medium 1651: MA medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 50179 SPEC: Aseptically transfer 0.1 ml of the culture to 5.0 ml of fresh medium in a 16 x 125 mm screw-capped test tube. Incubate cultures upright at 25C with the caps on loosely. Transfer cultures every two weeks.
Subcultivation
Protocol: ATCCNO: 50179 SPEC: Aseptically transfer 0.1 ml of the culture to 5.0 ml of fresh medium in a 16 x 125 mm screw-capped test tube. Incubate cultures upright at 25C with the caps on loosely. Transfer cultures every two weeks.
Cryopreservation

1.  Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.

2.  Adjust the concentration of cells to 2 x 106 - 2 x 107/ml in fresh medium.

3.  While cells are centrifuging prepare a 22% (v/v) solution of sterile DMSO in fresh medium.

4. Mix the cell preparation and the 10% methanol in equal portions. Thus, the final concentration will be 106 - 107 cells/ml and 11% (v/v) DMSO. The time from the mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.

5.  Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6.   Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at        -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately

      -1°C/min.)  

7.  The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.

Name of Depositor AT Soldo, EB Small
Chain of Custody
ATCC <<--AT Soldo, EB Small<<--G. Holz
References

Gomez-Saladin E, Small EB. Oral morphogenesis of the microstome to macrostome transformation in Miamiensis avidus Strain Ma/2. J. Eukaryot. Microbiol. 40: 363-370, 1993.

Gomez-Saladin E, Small E. Prey induced transformation of Miamiensis avidus strain Ma/2 by a soluble factor. J. Eukaryot. Microbiol. 40: 550-556, 1993.