Miamiensis avidus Thompson and Moewus (ATCC® 50180)

Strain Designations: Ma/2  /  Depositor: AT Soldo, EB Small  /  Biosafety Level: 1

Permits and Restrictions

View Permits

Strain Designations Ma/2
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format test tube
Type Strain no
Comments
Oral morphogenesis
Prey-induced transformation
Medium ATCC® Medium 1651: MA medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 50179 SPEC: Aseptically transfer 0.1 ml of the culture to 5.0 ml of fresh medium in a 16 x 125 mm screw-capped test tube. Incubate cultures upright at 25C with the caps on loosely. Transfer cultures every two weeks.
Subcultivation
Protocol: ATCCNO: 50179 SPEC: Aseptically transfer 0.1 ml of the culture to 5.0 ml of fresh medium in a 16 x 125 mm screw-capped test tube. Incubate cultures upright at 25C with the caps on loosely. Transfer cultures every two weeks.
Cryopreservation

1.  Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.

2.  Adjust the concentration of cells to 2 x 106 - 2 x 107/ml in fresh medium.

3.  While cells are centrifuging prepare a 22% (v/v) solution of sterile DMSO in fresh medium.

4. Mix the cell preparation and the 10% methanol in equal portions. Thus, the final concentration will be 106 - 107 cells/ml and 11% (v/v) DMSO. The time from the mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.

5.  Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6.   Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at        -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately

      -1°C/min.)  

7.  The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.

Name of Depositor AT Soldo, EB Small
Chain of Custody
ATCC <<--AT Soldo, EB Small<<--G. Holz
References

Gomez-Saladin E, Small EB. Oral morphogenesis of the microstome to macrostome transformation in Miamiensis avidus Strain Ma/2. J. Eukaryot. Microbiol. 40: 363-370, 1993.

Gomez-Saladin E, Small E. Prey induced transformation of Miamiensis avidus strain Ma/2 by a soluble factor. J. Eukaryot. Microbiol. 40: 550-556, 1993.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation