Plasmodium falciparum Welch (ATCC® 50110)

Strain Designations: HB-2  /  Depositor: W Trager  /  Biosafety Level: 2

Strain Designations HB-2
Application
Emerging infectious disease research
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
derived from existing strain
Isolation date: 1981
Product Format frozen
Type Strain no
Comments
This is a non-gametocyte-forming strain.
Medium ATCC® Medium 2196: Malaria medium, complete
Growth Conditions
Temperature: 37.0°C
Growth condition: in vitro culture in human erythrocytes. Consult product sheet for protocol.
Cryopreservation

CRYOPRESERVATION: 

        Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.

1.     Centrifuge ring stage culture for 5 min at 1800rpm in 50 ml centrifuge tube.

2.     Aspirate supernatant using sterile Pasteur pipet.

3.     Resuspend pellet gently in remaining supernatant.

4.     Slowly add 5 volumes of glycerolyte medium (see below) to 3 volumes pellet dropwise via a syringe as follows:

        A.    Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.

B.    Add the remaining 4 volumes of glycerolyte and gently agitate.

5.     Aliquot mixture into Nunc screwtop freezing vials and store at –80oC overnight.

6.     Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor.

** To formulate glycerolyte medium, combine the following with distilled water to 100 ml:  57.00g glycerol, 1.60g sodium lactate (C3H5NaO3), 30.00mg potassium chloride (KCl), 1.38g sodium dihydrogen phosphate (NaH2PO4). Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave to sterilize, and store at 4oC.

Name of Depositor W Trager
Special Collection ATCC
Year of Origin 1981
References

Bhasin VK, Trager W. Gametocyte-forming and non-gametocyte-forming clones of Plasmodium falciparum. Am. J. Trop. Med. Hyg. 33: 534-537, 1984. PubMed: 6383092