pSP65:pUAS14#4 plasmid in E. coli (ATCC® 45117)

Organism: Apple scar skin viroid  /  Clone Type: Clone  /  Depositors: AF Hadidi

Permits and Restrictions

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Designations pSP65:pUAS14#4 plasmid in E. coli
GenBank Number


Species Apple scar skin viroid
Depositors AF Hadidi
Construct size (kb): 3.3
Insert lengths(kb): 0.274
Insert Size (kb) 0.274
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.3; PstI--3.3; BglI--3.3; HindIII--3.3.
Derived from nucleotides 286 to 228 in the sequence of the circular viroid.
If the plasmid is linearized with PstI or HindIII, transcription with SP6 polymerase will generate the complement (minus strand).
Classification Aspcaviroid

Hashimoto J, Koganezawa H. Nucleotide sequence and secondary structure of apple scar skin viroid. Nucleic Acids Res. 15: 7045-7051, 1987. PubMed: 3658673

Ahmed F Hadidi, personal communication