pBW1085 (ATCC® 45099)

Organism: Beet mild yellowing virus  /  Clone Type: Clone  /  Depositors: BW Falk

Permits and Restrictions

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Designations pBW1085
Species Beet mild yellowing virus
Depositors BW Falk
Construct size (kb): 3.299999952316284
Insert lengths(kb): 0.5899999737739563
Gene product: genome, partial
Insert Size (kb) 0.590
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): PstI--2.7, 0.6; HindIII--3.3; BamHI--3.3.
This clone hybridizes with the 6.0 kb virion RNA from all beet western yellows luteovirus (BWYV) isolates tested, including some from Florida, California, and Idaho. It did not hybridize with other luteoviruses, including BYDV, PLRV, and SCRLV.
Classification Luteovirus

Falk BW, et al. Complementary DNA cloning and hybridization analysis of beet western yellows luteovirus RNAs. J. Gen. Virol. 70: 1301-1309, 1989.