Permits and Restrictions

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Designation / Description PTPA25 PLASMID IN ESCHERICHIA COLI JA221
U.S. Patent Number
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): BamHI--6.6; BamHI/HindIII--3.5, 3.1; HindIII--6.6.
Insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.25, 2.1; EcoRI--0.7, 1.2; PstI--0.55, 0.6, 1.25, 1.3; PvuII--1.25, 1.3, 1.6; SacI--0.05, 1.35; SmaI--1.65; StuI--1.4.
The 3' end of the insert contains the SV40 small t antigen splice site.
Shipped freeze-dried
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation

Wallner BP, et al. DNA sequences, recombinant DNA molecules and processes for producing human phospholipase inhibitor-like polypeptides. US Patent 4,874,743 dated Oct 17 1989