pMDIII (ATCC® 37502)

Applications: expression vectorshuttle vector  /  Depositors: H Wolf

Designations pMDIII
Permits and Restrictions

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Depositors H Wolf
Biosafety Level 1
Vector Information
Size (kb): 7.0000000000000000
Vector: pMDIII (plasmid)
Promoters: Promoter SV40 early
Construct size (kb): 7.0
Features: marker(s): ampR
promoter: SV40 early
replicon: pMB1
enhancer: SV40
expression vector
shuttle vector
A vector for expression of cloned sequences under the control of the SV40 early promoter in mammalian cell culture selected for dhfr+ phenotype.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Breathnach R, Harris BA. Plasmids for the cloning and expression of full-length double-stranded cDNAs under the control of the SV40 early or late gene promoter. Nucleic Acids Res. 11: 7119-7136, 1983. PubMed: 6314276

Kaufman RJ, Sharp PA. Construction of a modular dihydrofolate reductase cDNA gene: analysis of signals utilized for efficient expression. Mol. Cell. Biol. 2: 1304-1319, 1982. PubMed: 6131378

Motz M, et al. Expression of the Epstein-Barr virus major membrane protein in Chinese hamster ovary cells. Gene 44: 353-359, 1986. PubMed: 3023198

Shipped freeze-dried