pICEM19H- (ATCC® 37378)

Applications: vector permitting production of single-stranded DNA  /  Depositors: J Marsh

Designations pICEM19H-
Permits and Restrictions

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Depositors J Marsh
Biosafety Level 1
Vector Information
Size (kb): 3.9730000495910650
Vector: pICEM19H- (phagemid)
Construction: pIC7, pEMBL8-
Construct size (kb): 3.973000049591065
Features: insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
vector permitting production of single-stranded DNA
Streak to xgal + amp plate and pick a blue colony.
Phagemid with polylinker in lacZ' for production of ssDNA.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Marsh JL, et al. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32: 481-485, 1984. PubMed: 6099327

Shipped freeze-dried