ptac12 (ATCC® 37138)

Applications: contains easily purifiable cassette(s) for constructionexpression vector  /  Depositors: E Amann

Designations ptac12
Permits and Restrictions

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Depositors E Amann
Biosafety Level 1
Vector Information
Size (kb): 2.60
Vector: ptac12 (plasmid)
Promoters: Promoter tac
Construction: pEA300 (ATCC 37181), pGL101
Marker(s):ampR
Construct size (kb): 2.6
Features: marker(s): ampR
promoter: tac
replicon: pMB1
enhancer: none
Applications
contains easily purifiable cassette(s) for construction
expression vector
Comments
A 55 bp HpaII/PvuII fragment containing the -10 region of the lacUV5 promoter was inserted into the ClaI site of pEA300 (ATCC 37181).
Contains the lac operator. The PvuII site is located 5 bp downstream of the lacZ ribosome binding sequence.
The tac promoter can be excised by a EcoRI/PvuII digest.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Amann E, et al. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25: 167-178, 1983. PubMed: 6363212

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid.