YEp24 [pRB5] (ATCC® 37051)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector for constructing genomic libraries  /  Depositors: D Botstein

Permits and Restrictions

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Designations YEp24 [pRB5]
Depositors D Botstein
Biosafety Level 1
Vector Information
Size (kb): 7.7690000534057620
Vector: YEp24 (plasmid)
Promoters: Promoter none
Construction: pBR322, Saccharomyces cerevisiae, 2 micron ori
Marker(s):URA3,ampR,tetR
Construct size (kb): 7.769000053405762
Features: marker(s): URA3
marker(s): ampR
marker(s): tetR
promoter: none
replicon: 2 micron
replicon: pMB1
terminator: none
enhancer: none
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector for constructing genomic libraries
Comments
YE-type episomal shuttle vector.
Plasmid was constructed by inserting a 2.2 kb EcoRI 2 micron B fragment and a 1.1 kb HindIII URA3 fragment into the EcoRI and HindIII sites of pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Related Products
component of:ATCC 87635
Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37051