pBR325Amy (ATCC® 31792)

Organism: Geobacillus stearothermophilus (Donk) Nazina et al.  /  Clone Type: Clone  /  Depositors: CPC International, Inc.

Permits and Restrictions

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Designations pBR325Amy
Species Geobacillus stearothermophilus (Donk) Nazina et al.
Depositors CPC International, Inc.
Applications
produces protein amylase, alpha 1A; salivary Amylase, salivary, alpha-1A
Vector
Construct size (kb): 11.19999980926514
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR, tetR
replicon: pMB1
Cross references:
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR, tetR
replicon: pMB1
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 5.4
Cross references:
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 5.4
Cross references:
Insert lengths(kb): 5.400000095367432
Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A)
Target Gene: amylase, alpha 1A; salivary
Insert Size (kb) 5.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Plasmid expresses a thermostable alpha-amylase.
References

Mielenz JR, Mickel S. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis. US Patent 4,493,893 dated Jan 15 1985

Bascic Documentation
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Product Sheet
Product Sheet
Product Sheet