Tetrahymena pyriformis (Ehrenberg) Lwoff (ATCC® 30005)

Strain Designations: E (amicronucleate)  /  Depositor: AM Elliott  /  Biosafety Level: 1

Permits and Restrictions

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Strain Designations E (amicronucleate)
electrophoretic characterization
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Van Cortland Park, NY, 1932
Product Format test tube
Type Strain yes
electrophoretic characterization
Medium ATCC® Medium 357: Tetrahymena medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
1.  Transfer Tetrahymena from usual growth medium to ATCC Medium 1034 and allow to grow to near peak density.

2.   Harvest cells from a culture by centrifugation at 300 x g for 2 min.          

3.   Adjust concentration of cells to 2 x 106/ml in fresh


4.   While cells are centrifuging, prepare a 22% (v/v) sterile

solution of sterile DMSO in fresh medium.

a) Add 2.2 ml of DMSO to an ice cold 20 x 150 mm screw-capped test tube;

b) Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 7.8 ml of ice cold medium;

c) Invert several times to dissolve the DMSO;

d) Allow to warm to room temperature.

5.   Add a volume of the DMSO solution equal to the cell

      suspension volume but add in 3 equal aliquots at 2 min

      intervals. Thus, the final concentration of the preparation

      will equal 11% (v/v) DMSO and 106 cells /ml.

6.   Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic

      screw-capped cryules (special plastic vials for       cryopreservation).

7.   Place the ampules in a controlled rate freezing unit. The

cooling cycle should be initiated no less than 15 min and no longer than 60 min after the addition of the DMSO to the cell preparation. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At  -50°C ampules are plunged into liquid nitrogen.

8.   Store in the vapor or liquid phase of a nitrogen


9.   To establish a culture from the frozen state aseptically add 0.5 ml of sterile modified PYNFH medium (ATCC Medium 1034) containing 8% (w/v) sucrose to the ampule.  Immediately, place in a 35°C water bath, until thawed. Immerse the ampule just sufficient to cover the frozen material. Do not agitate the ampule.

10.          Immediately after thawing, aseptically remove the contents of the ampule and gently add the material to the edge of a 20 x 100 mm Petri plate containing ATCC Medium 919 (non-nutrient agar) and position on a 15 degree slant.  The cell suspension will pool at the edge of the plate.

11.          Continue to double the volume of the cell suspension at 10

minute intervals by adding ATCC medium 1034) containing 4% sucrose (w/v).  When the volume reaches 16.0 ml place the plate in horizontal position and incubate at 25°C. 

12.          On the following day, gently remove the cell suspension for the plate and transfer to a T-25 tissue culture flask.  Note the volume of the suspension and add a volume of fresh medium containing 4% sucrose equal to the volume of  the cell suspension.  Incubate the culture at 25°C.

13.          After culture has been established subculture into fresh

      normal medium without sucrose. 

Name of Depositor AM Elliott
Year of Origin 1932

Borden D, et al. Electrophoretic characterization of classical Tetrahymena pyriformis strains. J. Protozool. 20: 693-700, 1973. PubMed: 4148695

Biol. Bull. 65: 45-56, 1933.

Elliott AM. A quarter century exploring Tetrahymena. J. Protozool. 6: 1-7, 1959.

Slater JV. The magnesium requirement of Tetrahymena. Physiol. Zool. 25: 283-287, 1962.

Levy MR. Effects of inhibitors of RNA and protein synthesis on activation of glyconeogenesis in Tetrahymena. J. Cell. Physiol. 69: 247-252, 1967. PubMed: 6033953

Gross JA. A comparison of different criteria for determining the effects of antibiotics on Tetrahymena pyriformis E. J. Protozool. 2: 42-47, 1955.

Levy MR. Interaction of acetate, glucose and growth conditions on glyconeogenesis and isocitrate lyase activity in Tetrahymena. Comp. Biochem. Physiol. 21: 291-298, 1967. PubMed: 6036928

Muller M, et al. Distribution of tricarboxylic acid cycle enzymes and glyoxylate cycle enzymes between mitochondria and peroxisomes in Tetrahymena pyriformis. J. Biol. Chem. 243: 5385-5395, 1968. PubMed: 4387403

type strain

Jaso-Friedmann L, et al. Role of nonspecific cytotoxic cells in the induction of programmed cell death of pathogenic protozoans: participation of the Fas ligand-Fas receptor system. Exp. Parasitol. 96: 75-88, 2000. PubMed: 11052866

Cross References

Nucleotide (GenBank) : X92714 T.pyriformis gapC gene.

Nucleotide (GenBank) : X99544 T.pyriformis gapC gene.

Nucleotide (GenBank) : X99629 T.pyriformis mRNA for NAD-dependent glyceraldehyde-3-phosphate dehydrogenase.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation