Genomic RNA isolated from a preparation of Severe acute respiratory syndrome-related coronavirus 2 isolate USA/CA_CDC_5574/2020.
This isolate is lineage B.1.1.7, clade GR according to GISAID (Global Initiative on Sharing All Influenza Data) conventions.
The following mutations were identified in the clinical isolate: Spike A570D, Spike D614G, Spike D1118H, Spike H69del, Spike N501Y, Spike P681H, Spike S982A, Spike T716I, Spike V70del, Spike Y145del, M (Membrane protein) V70L, N (Nucleocapsid protein) D3L, N G204R, N R203K, N S235F, NS3 T223I, NS8 (Non-structural protein 8) Q27stop, NS8 R52I, NS8 Y73C, NSP3 (Non-structural protein 3) A890D, NSP3 I1412T, NSP3 T183I, NSP6 (Non-structural protein 6) F108del, NSP6 G107del, NSP6 S106del, NSP12 (Non-structural protein 12) P323L, NSP13 (Non-structural protein 13) A454V, NSP13 K460R.
One additional SNP in ORF1ab L3826F was reported in the deposited passage two virus, in comparison to the clinical specimen.
Genomic RNA from SARS-CoV-2 must be handled at BSL-2 containment.
Innocuity testing was performed on the extracted RNA and results reported on the Certificate of Analysis.