VH 2 (ATCC® CCL-140)

Organism: Vipera russelli, Russell's viper  / 

Permits and Restrictions

View Permits

Organism Vipera russelli, Russell's viper
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 35; range = 22 to 67.
Karyotype stable with a near-diploid stemline number. The cells have 16 macrochromosomes and from 17 to 20 microchromosomes.
Clinical Data
female
Virus Susceptibility Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus
Human poliovirus 1
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. .
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 30°C in a closed flask without CO2 in air atmosphere.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Once per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 30.0°C
Name of Depositor HF Clark
Year of Origin June, 1968
References

. . In Vitro 6: 376, 1971.

Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . In Vitro 6: 376, 1971.

Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139