XTT Cell Proliferation Assay Kit (ATCC® 30-1011K)

Permits and Restrictions

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Product Format frozen 1000 assays
Storage Conditions -20°C in the dark
Applications

A convenient system used to measure cell growth and drug sensitivity in tumor cell lines.

Tetrazolium salts have been widely used as detection reagents for many years in histochemical localization studies and cell biology assays (1,2). The second generation tetrazolium dye, XTT, can be effectively used in cell proliferation, cytotoxicity, and apoptosis assays (2,3,4). XTT is reduced to a soluble, brightly colored orange derivative by a mix of cellular effectors. The sensitivity of an XTT assay is greatly improved by the usage of an intermediate electron carrier, PMS (N-methyl dibenzopyrazine methyl sulfate). PMS helps drive XTT reduction and the formation of its formazan derivative.


Biosafety Level 1
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation