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C-33 A (ATCC® CRM-HTB-31™)

Organism: Homo sapiens, human  /  Cell Type: epithelial cell (CRM)  /  Disease: Retinoblastoma

Organism Homo sapiens, human
Cell Type epithelial cell (CRM)
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Retinoblastoma
Age 66 years adult
Gender female
Ethnicity Caucasian
Applications
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009
Intended Use


Certified Reference Material produced under an ISO Guide 34:2009 accredited process. 


    ACLASS Accredited Reference Material Producer AR-1384
Storage Conditions liquid nitrogen vapor phase
Karyotype This a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 70% of cells examined. Polyploid cells occurred at 8.6%. Seven marker chromosomes were consistently detected per pseudodiploid cell. They are: t(1q17q), t(1p21q), del(18) (q21.3), der(1)t(1;17) (p16;q21.3) and three others. Several other markers were also found but they occurred only once in 15 metaphases analyzed. Neither DMs nor HSRs were detected. Structurally normal N1 was absent. Generally there are two X chromosomes in each cell.
Derivation
The C-33 A cell line is one of a series of lines (see also ATCC CRL-1594 and ATCC CRL-1595) derived by N. Auersperg from cervical cancer biopsies.
Clinical Data
female
Caucasian
66 years adult
Oncogene p53 +; pRB +
Genes Expressed
p53 +; pRB +
Tumorigenic YES
Effects
Yes, in nude mice; forms undifferentiated carcinoma
Comments

Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

The line exhibited a hypodiploid karyotype initially, and an epithelial morphology. Karyological instability was observed with continued passage. The retinoblastoma protein (pRB) is present but abnormal in size.

Expression of p53 is elevated, and there is a point mutation at codon 273 resulting in a Arg -> Cys substitution. The cells are negative for human papillomavirus

Item can be used as a negative control; HPV-16 is not detected

 
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Cryopreservation
Freeze Medium: Culture medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 13
D16S539: 13,14
D5S818: 11,12
D7S820: 10
THO1: 7,8
TPOX: 9
vWA: 18,20
Isoenzymes
AK-1, 1
ES-D, 1
Name of Depositor N Auersperg
Deposited As Homo sapiens
Passage History
The C-33 A cell line is one of a series of lines (see also ATCC CRL-1594 and ATCC CRL-1595) derived by N. Auersperg from cervical cancer biopsies. The line exhibited a hypodiploid karyotype initially, and an epithelial morphology. Karyological instability was observed with continued passage. The retinoblastoma protein (pRB) is present but abnormal in size. Expression of p53 is elevated, and there is a point mutation at codon 273 resulting in a Arg -> Cys substitution. The cells are negative for human papillomavirus
References

J. Natl. Cancer Inst. 32: 135-148, 1964.

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

Kovelman R, et al. Enhanced transcriptional activation by E2 proteins from the oncogenic human papillomaviruses. J. Virol. 70: 7549-7560, 1996. PubMed: 8892874

Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
FAQ's
  1. Agreement to transfer to a CRO


    Date Updated: 1/2/2013

References

J. Natl. Cancer Inst. 32: 135-148, 1964.

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

Kovelman R, et al. Enhanced transcriptional activation by E2 proteins from the oncogenic human papillomaviruses. J. Virol. 70: 7549-7560, 1996. PubMed: 8892874