Derivation
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The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line ( ATCC® HTB-37™) by limiting dilution.
The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.
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Receptor Expression
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epidermal growth factor (EGF)
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Comments
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The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.
C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon.
Isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II.
The cells express substantial levels of BB myosin I similar to that of the human enterocyte.
Although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.
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