pUB110cop1 (ATCC® 77133)

Applications: shuttle vectorvector with low copy number  /  Depositors: H Leonhardt

Designations pUB110cop1
Permits and Restrictions

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Depositors H Leonhardt
Biosafety Level 1
Vector Information
Size (kb): 4.5000000000000000
Vector: pUB110cop1 (plasmid)
Promoters: Promoter none
Construction: pUB110, pEB114cop1
Construct size (kb): 4.5
Features: marker(s): kanR, neoR
promoter: none
replicon: pUB110
shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; NcoI--4.5; BamHI--4.5.
The copy number is 11 +/- 2 in Bacillus subtilis, resulting in an increase in structural and segregational stability.
Low copy number shuttle vector.
Constructed by replacing the NcoI/AflII fragment of pUB110 (nt 4033-4543) with the corresponding fragment from pEB114cop1 which contains a G -> T transversion in the Shine-Dalgarno sequence of repU at position 4130.
Media ATCC Medium 1065 (see below) plus kanamycin/neomycin (3 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C

Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061

Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622

Leonhardt H. Identification of a low-copy-number mutation within the pUB110 replicon and its effect on plasmid stability in Bacillus subtilis. Gene 94: 121-124, 1990. PubMed: 2121616

Shipped freeze-dried